Day 1: Assuming this does not follow directly from experiment 1, I would have had to prepare agar plates specifically for this experiment. Both halves of this lab use LB agar plates, which are the powder needed to mix with water to create the concentration required for the Petri dishes. If you'd like to know how I made them, please take a look at Experiment 1. However, in additon to preparing the agar plates, we also need to take out our freeze-dried PJE202 luminescent bacteria out from the freezer. The first step was to add sterile water into the tube, so I added 4 drops of it into the PJE202, closed the lid, and shook it for about a minute.Â